Digital Protein Detection at Single-Molecule Resolution
Overview
Protevera™ is a next-generation digital protein detection platform that enables ultra-sensitive, near-single-molecule detection and absolute-like quantification of protein biomarkers.
Built on a proprietary proximity-based molecular amplification chemistry, Protevera™ transforms protein detection into a digital counting problem, delivering performance comparable to — and, in key aspects, surpassing — existing ultra-sensitive platforms.
How It Works
Protevera™ utilizes a binding-induced nucleic acid amplification strategy:
Target proteins are captured by paired proximity probes
Binding events trigger oligonucleotide extension reactions
Signal molecules are amplified and partitioned
Digital readout is performed using dPCR systems
This converts protein detection into high-precision digital quantification.
Key Advantages
Ultrasensitive Detection
Detection limits below 10 fg/mL (attomolar range)
Near Single Molecule Resolution
True digital detection of low-abundance biomarkers
Wash-Free Workflow
Simplified operation, minimal hands-on time
Ultra-Low Sample Input
Requires only 1–5 μL sample volume
High Compatibility
Runs on standard digital PCR instruments
Cost-Effective Alternative
Significantly lower cost compared to bead-based single-molecule systems
Rapid Customization
New targets can be developed within hours to days
Performance
Super sensitivity and specificity
Steady detection of trace biomarkers in the blood.
Performance:
LOD down to ~0.1 fM (fg/mL level) for cytokines and NFL
Comparable performance in single-plex and multiplex formats
High specificity with minimal cross-reactivity
Strong clinical discrimination performance (e.g., pTau217 AUC ~0.95)
Multiplex Without Trade-Offs
Protevera™ enables true multiplex protein detection:
Maintain sensitivity across multiple targets
Minimal signal interference
Suitable for panel-based biomarker profiling
Applications
Neurology
Alzheimer’s disease biomarkers (pTau217, NFL)
Oncology
Circulating protein biomarkers, minimal residual disease monitoring
Immunology
Cytokines (IL-6, IFN-γ, TNF-α) and immune profiling
Infectious Disease
Low-abundance pathogen-associated proteins
Why Protevera™
Digital sensitivity. PCR scalability. Protein-level insight.
Protevera™ delivers a scalable, instrument-compatible, and cost-efficient alternative to traditional ultra-sensitive protein-detection platforms, enabling broader adoption across research labs and clinical diagnostics.