High-Multiplex PCR + Melting Curve 2D Coding Platform
Overview
MeltArray™ is a high-multiplex PCR platform designed to break the traditional multiplex ceiling of real-time PCR. By combining fluorophore channel coding with melting temperature (Tm) signature analysis, MeltArray enables dozens of targets to be detected in a single reaction tube using a standard real-time PCR instrument.
Scientific Foundation
MeltArray™ expands traditional multiplex PCR capacity by encoding targets through distinct melting curve signatures rather than fluorescence channel limitation. The platform design enables broad pathogen coverage without increasing instrument complexity.
Key Features:
High-level multiplexing (dozens of targets per reaction)
Rapid turnaround time (typically <3 hours)
Single-tube detection workflow
Quantitative Ct and qualitative Tm dual-readout system
Core Technical Capabilities
Up to 72 targets per reaction (platform capacity)
Dozens of mutations detectable in a single tube
2D coding: Fluorophore channel + melting temperature signature
Compatible with standard 4-channel real-time PCR systems
Analytical Performance
Limit of detection (LOD): 0.05%–0.5% mutant allele fraction (MAF)
Mutation enrichment: 23.5× to 59.4×
SNV discrimination with high melting curve resolution
Artifact suppression using UNG pretreatment
Workflow Efficiency
Total turnaround time: <5 hours
Hands-on time: ~20 minutes
>90 samples per 96-well run
Single-tube pretreatment + single-tube detection
Clinical & Operational Advantages
Designed for FFPE tumor DNA workflows
Eliminates formalin-induced sequencing artifacts
Enables rapid therapy selection decisions
Cost-effective alternative to NGS for hotspot panels
Reference: Q. Huang et al, Highly multiplex PCR assays by coupling the 5′-flap endonuclease activity of Taq DNA polymerase and molecular beacon reporters, Proc. Natl. Acad. Sci. U.S.A. 119 (9) e2110672119, (2022).